Hotspot

psbA

LSC · 81-1142 · 1062 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGGGTATTTCTGGCTTTCCT	GTATGCTTGGGAGTCCTTGC	45.0	55.0	56.4	58.6	1162 bp	1.0	62.3
psbA_p2	TGGGTATTTCTGGCTTTCCT	TGGGAGTCCTTGCAATTTGA	45.0	45.0	56.4	57.3	1155 bp	1.0	61.8
psbA_p3	TGGGTATTTCTGGCTTTCCT	GCTTGGGAGTCCTTGCAATT	45.0	50.0	56.4	58.7	1158 bp	1.0	61.3
psbA_p4	TGGGTATTTCTGGCTTTCCT	TGTATGCTTGGGAGTCCTTG	45.0	50.0	56.4	57.2	1163 bp	1.0	60.9
psbA_p5	TGGGTATTTCTGGCTTTCCTT	GTATGCTTGGGAGTCCTTGC	42.9	55.0	57.2	58.6	1162 bp	1.0	60.5