Hotspot

ndhF

SSC · 100114-102330 · 2217 bp

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Pi 0.000451

MarkerSeek score 57.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 1
pi: 0.000451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	58.2	58.1	2344-2345 bp	1.0	70.2
ndhF_p2	TGCATTTTGATGAGCCTCGT	GGTTTGGTGAATCGGAAACA	45.0	45.0	58.2	56.5	2343-2344 bp	1.0	67.0
ndhF_p3	TGCATTTTGATGAGCCTCGT	GGTTTGGTGAATCGGAAACAA	45.0	42.9	58.2	57.3	2343-2344 bp	1.0	64.9
ndhF_p4	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAAC	45.0	45.0	58.2	56.2	2344-2345 bp	1.0	64.6
ndhF_p5	TTGCATTTTGATGAGCCTCG	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.1	58.1	2345-2346 bp	1.0	64.2