Hotspot

atpA

LSC · 34026-35549 · 1524 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TTCCCCTTGCTTCCGAAAAA	TCCTCTTCTACTAACACGAGT	45.0	42.9	57.9	55.2	1611 bp	1.0	49.1
atpA_p2	TTTCCCCTTGCTTCCGAAAA	TCCTCTTCTACTAACACGAGT	45.0	42.9	57.9	55.2	1612 bp	1.0	49.1
atpA_p3	TTTTCCCCTTGCTTCCGAAA	TCCTCTTCTACTAACACGAGT	45.0	42.9	57.9	55.2	1613 bp	1.0	49.1
atpA_p4	TTTTTCCCCTTGCTTCCGAA	TCCTCTTCTACTAACACGAGT	45.0	42.9	57.9	55.2	1614 bp	1.0	49.1
atpA_p5	TTCCCCTTGCTTCCGAAAAA	TCCTCTTCTACTAACACGAGTT	45.0	40.9	57.9	56.0	1611 bp	1.0	47.4