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rpl32-trnL-UAG

SSC · 103401-103919 · 519 bp

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Pi 0.00193

MarkerSeek score 58.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 519
variable_sites: 1
pi: 0.00193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAACGAGCATTCAAAACCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	596 bp	1.0	78.1
rpl32-trnL-UAG_p2	GCAACGAGCATTCAAAACCA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.9	57.8	589 bp	1.0	77.6
rpl32-trnL-UAG_p3	GCAACGAGCATTCAAAACCA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.9	58.2	610 bp	1.0	77.6
rpl32-trnL-UAG_p4	GCAACGAGCATTCAAAACCA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.9	57.8	595 bp	1.0	77.5
rpl32-trnL-UAG_p5	GCAACGAGCATTCAAAACCA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.9	57.8	594 bp	1.0	77.5