Hotspot

ndhF-rpl32

SSC · 102331-103235 · 905 bp

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Pi 0

MarkerSeek score 58.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 905
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGAAAAAGCCCAAACCCCA	TGACATCGAAGTACGCTTTT	45.0	40.0	58.1	55.4	1021-1022 bp	1.0	58.7
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGCTTTT	55.0	40.0	58.1	55.4	987-988 bp	1.0	58.7
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGCTTT	55.0	40.0	58.1	55.4	988-989 bp	1.0	58.7
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGCTT	55.0	40.0	58.1	55.4	989-990 bp	1.0	58.7
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGCT	55.0	40.0	58.1	55.4	990-991 bp	1.0	58.7