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trnR-UCU-rps14

LSC · 35760-36138 · 379 bp

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Pi 0.00264

MarkerSeek score 55.70

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 1
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	ATGCATGTTTGTTACCGGGT	47.6	45.0	56.9	58.1	456 bp	1.0	63.1
trnR-UCU-rps14_p2	AGAAGACCTCTGTCCTATCCA	TCCGGACACATACTTCGAGA	47.6	50.0	56.9	57.9	484 bp	1.0	62.8
trnR-UCU-rps14_p3	AGGTTTAGAAGACCTCTGTCCT	ATGCATGTTTGTTACCGGGT	45.5	45.0	57.9	58.1	462 bp	1.0	62.4
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	445 bp	1.0	62.3
trnR-UCU-rps14_p5	AGGTTTAGAAGACCTCTGTCCT	TCCGGACACATACTTCGAGA	45.5	50.0	57.9	57.9	490 bp	1.0	62.1