Hotspot

trnG-UCC-trnT-GGU

LSC · 13176-14208 · 1033 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1033
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1123 bp 1.0 40.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT CCGTGGCACTACTCTACCA 38.1 57.9 55.5 58.4 1167 bp 1.0 40.3
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1119 bp 1.0 40.0
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1120 bp 1.0 40.0
trnG-UCC-trnT-GGU_p5 ACGAATCACACTTTTACCACT AGGAAAAGCCCTTTATCGGA 38.1 45.0 55.5 56.5 1145 bp 1.0 40.0