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rps16-trnQ-UUG

LSC · 5478-6230 · 753 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
753
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GTCGCAGAAAACGAAATGGG 45.0 50.0 58.1 58.0 890 bp 1.0 78.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 826 bp 1.0 78.3
rps16-trnQ-UUG_p3 TCATCTCCTTCAAGTCGCAC GTCGCAGAAAACGAAATGGG 50.0 50.0 57.6 58.0 901 bp 1.0 76.7
rps16-trnQ-UUG_p4 TCATCTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 837 bp 1.0 76.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 830 bp 1.0 73.5