Hotspot

rbcL-accD

LSC · 59286-60186 · 901 bp

Back to Triplostegia

Pi 0.00713
MarkerSeek score 63.70
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
901
variable_sites
6
pi
0.00713
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCGTTGGAGAGATCGTTTCT TGCTTGTATGAGGGAGTGGA 50.0 50.0 58.0 58.0 2416-2998 bp 1.0 84.1
rbcL-accD_p2 GCACGACTACTTAACAGGGG TGCTTGTATGAGGGAGTGGA 55.0 50.0 58.0 58.0 2254-2836 bp 1.0 84.1
rbcL-accD_p3 AATGAGGGACGTGATCTTGC TGCTTGTATGAGGGAGTGGA 50.0 50.0 58.0 58.0 1758-2340 bp 1.0 84.0
rbcL-accD_p4 ATGAAATTATCCGTGCGGCT TGCTTGTATGAGGGAGTGGA 45.0 50.0 58.0 58.0 1727-2309 bp 1.0 84.0
rbcL-accD_p5 GGTAGCTGCCGAATCTTCAA TCTCATCGCAACTCCGAATG 50.0 50.0 58.0 58.1 2529-2610 bp 1.0 83.5