Hotspot

trnE-UUC-trnT-GGU

LSC · 33441-34267 · 827 bp

Back to Triplostegia

Pi 0.0113

MarkerSeek score 64.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 9
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	904-914 bp	1.0	82.6
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	902-912 bp	1.0	82.6
trnE-UUC-trnT-GGU_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	883-893 bp	1.0	82.2
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	881-891 bp	1.0	82.2
trnE-UUC-trnT-GGU_p5	AACTTCCTTTCGCAGTACCC	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	965-975 bp	1.0	82.1