Hotspot

rps2-rpoC2

LSC · 17596-17897 · 302 bp

Back to Triplostegia

Pi 0.0232

MarkerSeek score 65.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 7
pi: 0.0232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AACTCCTGCCTCCATCATCT	ATTAGTGCACCCTTCAAGGC	50.0	50.0	58.1	58.2	462 bp	1.0	79.1
rps2-rpoC2_p2	TGCCTCCATCATCTCTTCCA	ATTAGTGCACCCTTCAAGGC	50.0	50.0	58.1	58.2	456 bp	1.0	79.0
rps2-rpoC2_p3	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	401 bp	1.0	78.5
rps2-rpoC2_p4	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	395 bp	1.0	78.5
rps2-rpoC2_p5	TCCTGCCTCCATCATCTCTT	ATTAGTGCACCCTTCAAGGC	50.0	50.0	57.8	58.2	459 bp	1.0	78.4