Hotspot

rps16-trnQ-UUG

LSC · 6216-7984 · 1769 bp

Back to Triplostegia

Pi 0.00299

MarkerSeek score 64.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1769
variable_sites: 5
pi: 0.00299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AGAGCACCCTCATTCCGATA	TCGGAGGTTCGAATCCTTTC	50.0	50.0	57.9	57.3	1817-1913 bp	1.0	78.2
rps16-trnQ-UUG_p2	AGAGCACCCTCATTCCGATA	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.9	58.7	1844-1940 bp	1.0	78.1
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TCGGAGGTTCGAATCCTTTC	45.0	50.0	58.1	57.3	1749-1845 bp	1.0	77.8
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1776-1872 bp	1.0	77.7
rps16-trnQ-UUG_p5	AGAGCACCCTCATTCCGATA	GGGTTTTGGTCCCGCTATTC	50.0	55.0	57.9	58.9	1835-1931 bp	1.0	76.3