Hotspot

psaJ-rpl33

LSC · 67444-67801 · 358 bp

Back to Tripidium

Pi 0.0112

MarkerSeek score 45.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 358
variable_sites: 6
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	CCCCGTCGATTCCTTATTGG	45.0	55.0	57.9	58.1	520-528 bp	1.0	79.2
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	GAAATCCCCGTCGATTCCTT	45.0	50.0	57.9	57.7	525-533 bp	1.0	77.5
psaJ-rpl33_p3	ATGGTTTGGGGCTTTAGCAG	CCCCGTCGATTCCTTATTGG	50.0	55.0	58.4	58.1	524-532 bp	1.0	76.4
psaJ-rpl33_p4	TTTGGGGCTTTAGCAGGTTT	AGAAATCCCCGTCGATTCCT	45.0	50.0	57.9	58.5	526-534 bp	1.0	76.1
psaJ-rpl33_p5	TTTGGGGCTTTAGCAGGTTT	CCCGTCGATTCCTTATTGGT	45.0	50.0	57.9	57.4	519-527 bp	1.0	75.2