Hotspot

rps16

LSC · 4863-6056 · 1194 bp

Back to Triphora

Pi 0.0213

MarkerSeek score 60.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1194
variable_sites: 25
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGAATATAAAACAAACTTTGAAGAAAG	ACGGTTCCAAAATAAAATGAGAGG	25.9	37.5	54.6	57.7	1263-1309 bp	1.0	42.9
rps16_p2	GGAATATAAAACAAACTTTGAAGAAAG	AAATTGCATACGGTTCCAAA	25.9	35.0	54.6	54.1	1272-1318 bp	1.0	42.8
rps16_p3	GGAATATAAAACAAACTTTGAAGAAAG	AATTGCATACGGTTCCAAAA	25.9	35.0	54.6	54.1	1271-1317 bp	1.0	42.8
rps16_p4	GGAATATAAAACAAACTTTGAAGAAAG	AAATTGCATACGGTTCCAAAA	25.9	33.3	54.6	54.9	1272-1318 bp	1.0	42.8
rps16_p5	GGAATATAAAACAAACTTTGAAGAAAG	ATTGCATACGGTTCCAAAAT	25.9	35.0	54.6	53.8	1270-1316 bp	1.0	42.8