Hotspot

clpP

LSC · 67196-69688 · 2493 bp

Back to Triphora

Pi 0.0139

MarkerSeek score 63.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2493
variable_sites: 33
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGGTTTTGGCTTAGATCGA	AAACCATTGTTACGTTTCCA	45.0	35.0	56.8	53.6	2551-2653 bp	1.0	44.6
clpP_p2	TGGGTTTTGGCTTAGATCGA	AAAACCATTGTTACGTTTCCA	45.0	33.3	56.8	54.5	2552-2654 bp	1.0	44.6
clpP_p3	TGGGTTTTGGCTTAGATCGA	AACCATTGTTACGTTTCCAT	45.0	35.0	56.8	53.4	2550-2652 bp	1.0	44.6
clpP_p4	TGGGTTTTGGCTTAGATCGA	AAACCATTGTTACGTTTCCAT	45.0	33.3	56.8	54.3	2551-2653 bp	1.0	44.6
clpP_p5	TGGGTTTTGGCTTAGATCGAT	AAACCATTGTTACGTTTCCA	42.9	35.0	57.4	53.6	2551-2653 bp	1.0	44.6