Hotspot

rps8-rpl14

LSC · 78926-79156 · 231 bp

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Pi 0.0519
MarkerSeek score 73.90
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
231
variable_sites
12
pi
0.0519
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TCCCTTTTGATCCATGTAAGCA CGATTGCTCGGGAATTGAGA 40.9 50.0 57.7 58.1 365-376 bp 1.0 63.6
rps8-rpl14_p2 TCCCTTTTGATCCATGTAAGCA TTGCTCGGGAATTGAGACAG 40.9 50.0 57.7 57.9 362-373 bp 1.0 63.3
rps8-rpl14_p3 TCCCTTTTGATCCATGTAAGCA GCTCGGGAATTGAGACAGTT 40.9 50.0 57.7 57.9 360-371 bp 1.0 63.3
rps8-rpl14_p4 TCCCTTTTGATCCATGTAAGCA AATAGTTTCCTTGGCACCCG 40.9 50.0 57.7 58.2 328-339 bp 1.0 63.0
rps8-rpl14_p5 TCCCTTTTGATCCATGTAAGCA AACTCGAATCTTTGGTGCGA 40.9 45.0 57.7 57.8 382-393 bp 1.0 62.7