Hotspot

clpP-psbB

LSC · 69689-70619 · 931 bp

Back to Triphora

Pi 0.0213

MarkerSeek score 74.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 931
variable_sites: 18
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	1012-1075 bp	1.0	83.8
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	1007-1070 bp	1.0	83.7
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	964-1027 bp	1.0	83.5
clpP-psbB_p4	CGCACTATACGTCAACCCAA	CAATCGACCGGGATCATTCA	50.0	50.0	58.0	57.8	1001-1064 bp	1.0	83.5
clpP-psbB_p5	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	959-1022 bp	1.0	83.4