Hotspot

psaJ-rpl33

LSC · 64198-64852 · 655 bp

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Pi 0.0349

MarkerSeek score 77.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 22
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCCTTGTCATTCCCCTT	TGGCATCCTTTCGAACACAA	50.0	45.0	57.9	58.0	751-761 bp	1.0	83.5
psaJ-rpl33_p2	GATGCCTTGTCATTCCCCTT	TTGGCATCCTTTCGAACACA	50.0	45.0	57.9	58.0	752-762 bp	1.0	83.5
psaJ-rpl33_p3	TGGTTTGGGTCTTTAGCAGG	TGGCATCCTTTCGAACACAA	50.0	45.0	57.7	58.0	802-812 bp	1.0	82.1
psaJ-rpl33_p4	TGGTTTGGGTCTTTAGCAGG	TTGGCATCCTTTCGAACACA	50.0	45.0	57.7	58.0	803-813 bp	1.0	82.1
psaJ-rpl33_p5	GGTTTGGGTCTTTAGCAGGT	TGGCATCCTTTCGAACACAA	50.0	45.0	57.7	58.0	801-811 bp	1.0	82.1