Hotspot

accD-psaI

LSC · 55547-56317 · 771 bp

Back to Triphora

Pi 0.027

MarkerSeek score 63.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 19
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGCTGAACCTAACGCCTAC	AAAGAAGCCATTGCGATTGC	50.0	45.0	57.9	58.0	1003-1066 bp	1.0	85.4
accD-psaI_p2	ACCCGTTTGATCGAGTATGC	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.1	58.0	1232-1295 bp	1.0	85.2
accD-psaI_p3	GCAAGAAGGGAGTTTGAGCT	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.1	58.0	1158-1221 bp	1.0	85.0
accD-psaI_p4	TTGCTGAACCTAACGCCTAC	TTGCTGAGACGGAAATACGG	50.0	50.0	57.9	58.0	1630-1631 bp	1.0	84.3
accD-psaI_p5	ACCCGTTTGATCGAGTATGC	TTGCTGAGACGGAAATACGG	50.0	50.0	58.1	58.0	1859-1860 bp	1.0	83.8