Hotspot

psbC-trnS-UGA

LSC · 35131-35266 · 136 bp

Back to Triphora

Pi 0.0376

MarkerSeek score 70.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 5
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	258 bp	1.0	81.0
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	238 bp	1.0	80.4
psbC-trnS-UGA_p3	AGCGGGGTTTGAAAAAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.2	58.0	295 bp	1.0	80.3
psbC-trnS-UGA_p4	AGCGGGGTTTGAAAAAGGAA	ATAGCCCCGGTCTTGAAAAC	45.0	50.0	58.2	57.9	275 bp	1.0	79.6
psbC-trnS-UGA_p5	CCTGTTCTTTCCATGACCCC	ATCTGGGAGAGATGGCTGAG	55.0	55.0	58.2	58.3	264 bp	1.0	78.9