Hotspot

trnE-UUC-trnT-GGU

LSC · 30790-31652 · 863 bp

Back to Triphora

Pi 0.0256

MarkerSeek score 63.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 21
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCTTTTTAGGGACCGGGGAT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1200-1772 bp	1.0	83.5
trnE-UUC-trnT-GGU_p2	GGACCGGGGATTTATGTTCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1191 bp	0.5	59.8
trnE-UUC-trnT-GGU_p3	GGGACCGGGGATTTATGTTC	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1192 bp	0.5	59.8
trnE-UUC-trnT-GGU_p4	GGACCGGGGATTTATGTTCC	CCCTTGCCTTATTCTCGCAT	55.0	50.0	58.0	58.0	1808 bp	0.5	59.6
trnE-UUC-trnT-GGU_p5	GGGACCGGGGATTTATGTTC	CCCTTGCCTTATTCTCGCAT	55.0	50.0	58.0	58.0	1809 bp	0.5	59.6