Hotspot

petN-psbM

LSC · 28616-29426 · 811 bp

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Pi 0.02

MarkerSeek score 71.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 16
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.2	57.9	984-986 bp	1.0	80.2
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTCTTCATTT	50.0	45.0	58.2	57.9	973-975 bp	1.0	80.2
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTCTTCATT	50.0	45.0	58.2	57.9	974-976 bp	1.0	80.2
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTCTTCAT	50.0	45.0	58.2	57.7	975-977 bp	1.0	78.4
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	GCTTATTGCTACTGCGCTCT	50.0	50.0	58.2	58.4	979-981 bp	1.0	77.6