Hotspot

trnS-GCU-trnG-UCC

LSC · 8166-9010 · 845 bp

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Pi 0.0302
MarkerSeek score 75.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
845
variable_sites
25
pi
0.0302
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AGAAATGGAAAGATCCCTTGACT 50.0 39.1 58.0 57.5 955-1210 bp 1.0 54.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AGAAATGGAAAGATCCCTTGACT 50.0 39.1 58.0 57.5 954-1209 bp 1.0 54.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 914-1169 bp 1.0 54.8
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 913-1168 bp 1.0 54.8
trnS-GCU-trnG-UCC_p5 AATCAAAGGGGTCGGAGAGA AGAAATGGAAAGATCCCTTGACT 50.0 39.1 58.0 57.5 1020 bp 0.5 31.4