Hotspot

rps16-trnQ-UUG

LSC · 6057-6804 · 748 bp

Back to Triphora

Pi 0.0337

MarkerSeek score 78.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 24
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCCAATCATGTCC	ACAGGTTTTGGTCCCGTTAC	50.0	50.0	57.9	58.0	845-867 bp	1.0	84.8
rps16-trnQ-UUG_p2	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	822-844 bp	1.0	84.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	ACAGGTTTTGGTCCCGTTAC	45.0	50.0	58.1	58.0	822-844 bp	1.0	84.3
rps16-trnQ-UUG_p4	CCACAGCCAATCATGTCCTT	ACAGGTTTTGGTCCCGTTAC	50.0	50.0	58.2	58.0	843-865 bp	1.0	84.0
rps16-trnQ-UUG_p5	ATCCACAGCCAATCATGTCC	TCTTTTAGGAATGGGGCGTG	50.0	50.0	57.9	57.9	881-903 bp	1.0	83.8