Hotspot

ycf1-trnN-GUU

IRa · 130889-131960 · 1072 bp

Back to Triosteum

Pi 0.252

MarkerSeek score 81.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1072
variable_sites: 465
pi: 0.252
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATGAACATCACGAGCTGTCC	CTCGACGCCAGGATGTTAAA	50.0	50.0	58.0	58.0	1867-2470 bp	1.0	91.8
ycf1-trnN-GUU_p2	GGACCCTATGCAGAATGTGG	CTCGACGCCAGGATGTTAAA	55.0	50.0	58.0	58.0	1766-2369 bp	1.0	91.7
ycf1-trnN-GUU_p3	ATGAACATCACGAGCTGTCC	CACATTCTGGTGTCCTAGGC	50.0	55.0	58.0	58.0	1976-2579 bp	1.0	91.6
ycf1-trnN-GUU_p4	TGTCATTATTCCCGCGGAAG	CTCGACGCCAGGATGTTAAA	50.0	50.0	58.1	58.0	1974-2577 bp	1.0	91.5
ycf1-trnN-GUU_p5	GGACCCTATGCAGAATGTGG	CACATTCTGGTGTCCTAGGC	55.0	55.0	58.0	58.0	1875-2478 bp	1.0	91.5