Hotspot

rpl32-trnL-UAG

SSC · 115163-115966 · 804 bp

Back to Triosteum

Pi 0.0252
MarkerSeek score 69.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
804
variable_sites
37
pi
0.0252
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAGTCA AGCGTGTCTACCGATTTCAC 36.4 50.0 57.1 58.0 829-889 bp 1.0 64.1
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAGTCA CAGCGTGTCTACCGATTTCA 36.4 50.0 57.1 58.0 830-890 bp 1.0 64.0
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAGTCA TCCTAAGAGCAGCGTGTCTA 36.4 50.0 57.1 57.9 839-899 bp 1.0 63.0
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAGTCA CCTAAGAGCAGCGTGTCTAC 36.4 55.0 57.1 57.8 838-898 bp 1.0 62.5
rpl32-trnL-UAG_p5 TGTGCGACAAACAAATAAGTCA CTAAGAGCAGCGTGTCTACC 36.4 55.0 57.1 57.8 837-897 bp 1.0 62.5