Hotspot

trnN-GUU-ndhF

IRb · 111777-112618 · 842 bp

Back to Triosteum

Pi 0.0817

MarkerSeek score 85.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 842
variable_sites: 118
pi: 0.0817
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TACCGCTAAGCTATTGGGGA	AACCAAAATCCCCAACACGA	50.0	45.0	57.9	57.9	1427-1714 bp	1.0	87.5
trnN-GUU-ndhF_p2	CCATTCGACCATATCCGCAT	AACCAAAATCCCCAACACGA	50.0	45.0	57.9	57.9	1600-2217 bp	1.0	86.8
trnN-GUU-ndhF_p3	CTCGACGCCAGGATGTTAAA	AAGCCCACATACGACGAAAA	50.0	45.0	58.0	57.8	1581-2198 bp	1.0	86.7
trnN-GUU-ndhF_p4	CTCGACGCCAGGATGTTAAA	AACCAAAATCCCCAACACGA	50.0	45.0	58.0	57.9	2022-2639 bp	1.0	86.1
trnN-GUU-ndhF_p5	CACATTCTGGTGTCCTAGGC	AACCAAAATCCCCAACACGA	55.0	45.0	58.0	57.9	2131-2748 bp	1.0	85.6