Hotspot

accD-psaI

LSC · 61980-62711 · 732 bp

Back to Triosteum

Pi 0.0454

MarkerSeek score 77.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 55
pi: 0.0454
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTGACAGCCAGTTTTGGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	867-1056 bp	1.0	88.8
accD-psaI_p2	ACATCTCCTACTACCGGTGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	887-1076 bp	1.0	88.4
accD-psaI_p3	GGTGACAGCCAGTTTTGGTA	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1407-1596 bp	1.0	87.5
accD-psaI_p4	GGTCCACGGTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	657-845 bp	0.75	78.1
accD-psaI_p5	GGTCCACGGTTTCTTTCCTT	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1197-1386 bp	0.75	76.6