Hotspot

rbcL-accD

LSC · 58637-60335 · 1699 bp

Back to Triosteum

Pi 0.194

MarkerSeek score 75.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1699
variable_sites: 363
pi: 0.194
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCACGACTACTTAACAGGGG	CTGAAAGCGAGACCACTACC	55.0	55.0	58.0	58.0	1855-2569 bp	1.0	91.9
rbcL-accD_p2	CGTAGCTAATCGAGTCGCTC	CTGAAAGCGAGACCACTACC	55.0	55.0	58.1	58.0	1402-2116 bp	1.0	91.5
rbcL-accD_p3	GCTCTGACCGAGATCTTTGG	CTGAAAGCGAGACCACTACC	55.0	55.0	58.1	58.0	1488-2202 bp	1.0	91.4
rbcL-accD_p4	AGATTGGGTCTCTCTACCGG	CTGAAAGCGAGACCACTACC	55.0	55.0	57.9	58.0	1555-2269 bp	1.0	91.4
rbcL-accD_p5	GCACGACTACTTAACAGGGG	ACAAGAACAACACTGCCCAT	55.0	45.0	58.0	57.9	1672-2386 bp	0.5	66.0