Hotspot

ndhK-ndhC

LSC · 51966-52025 · 60 bp

Back to Triosteum

Pi 0.0361

MarkerSeek score 72.60

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 4
pi: 0.0361
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	ACCATAGAGAAGCGGCCATA	AAAAGGGGCATTGGAATGGT	50.0	45.0	58.3	58.0	201-207 bp	1.0	80.9
ndhK-ndhC_p2	ACCATAGAGAAGCGGCCATA	GGTTCAGTTTATGCATGGCG	50.0	50.0	58.3	57.8	221-227 bp	1.0	79.4
ndhK-ndhC_p3	ACCATAGAGAAGCGGCCATA	CGAAAAGGGGCATTGGAATG	50.0	50.0	58.3	57.7	203-209 bp	1.0	78.8
ndhK-ndhC_p4	ACCATAGAGAAGCGGCCATA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.3	57.8	296-302 bp	1.0	78.6
ndhK-ndhC_p5	ACCATAGAGAAGCGGCCATA	AAAGGGGCATTGGAATGGTC	50.0	50.0	58.3	58.4	200-206 bp	1.0	77.9