Hotspot

psaA-ycf3

LSC · 43587-44356 · 770 bp

Back to Triosteum

Pi 0.0284

MarkerSeek score 82.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 36
pi: 0.0284
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCGGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	961-979 bp	1.0	84.8
psaA-ycf3_p2	TGAGAAATGACCGGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	943-961 bp	1.0	84.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	924-942 bp	1.0	84.8
psaA-ycf3_p4	TGAGAAATGACCGGGTTTGG	TTGGTTGAAGATCACCAGGC	50.0	50.0	58.1	58.1	894-912 bp	1.0	84.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	942-960 bp	1.0	84.7