primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10632	10805	245-257	1.000000	71.8
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10632	10805	248-260	1.000000	71.5
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10632	10805	251-263	1.000000	71.0
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10632	10805	254-266	1.000000	70.8
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10632	10805	249-261	1.000000	69.3
psaA-ycf3_p1	psaA-ycf3	1	TGAGAAATGACCGGGTTTGG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.1	58.0	6.3	0.0	0.136672	43587	44356	961-979	1.000000	84.8
psaA-ycf3_p2	psaA-ycf3	2	TGAGAAATGACCGGGTTTGG	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	58.1	58.0	6.3	18.7	0.140786	43587	44356	943-961	1.000000	84.8
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	43587	44356	924-942	1.000000	84.8
psaA-ycf3_p4	psaA-ycf3	4	TGAGAAATGACCGGGTTTGG	TTGGTTGAAGATCACCAGGC	20	20	50.0	50.0	58.1	58.1	6.3	3.2	0.186013	43587	44356	894-912	1.000000	84.8
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	43587	44356	942-960	1.000000	84.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	20	20	55.0	50.0	58.0	56.8	10.3	3.3	1.224095	50003	50711	837-853	1.000000	72.8
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	20	20	55.0	50.0	58.0	56.8	10.3	3.3	1.226313	50003	50711	836-852	1.000000	72.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	50003	50711	801-817	1.000000	72.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	50003	50711	800-816	1.000000	72.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CTGGTAGAGCAGAGGACTGA	TGGATAGGATGGCCTTTACG	20	20	55.0	50.0	57.9	56.8	2.5	3.3	1.357888	50003	50711	825-841	1.000000	71.8
ndhK-ndhC_p1	ndhK-ndhC	1	ACCATAGAGAAGCGGCCATA	AAAAGGGGCATTGGAATGGT	20	20	50.0	45.0	58.3	58.0	2.2	1.7	0.326275	51966	52025	201-207	1.000000	80.9
ndhK-ndhC_p2	ndhK-ndhC	2	ACCATAGAGAAGCGGCCATA	GGTTCAGTTTATGCATGGCG	20	20	50.0	50.0	58.3	57.8	2.2	10.2	0.476274	51966	52025	221-227	1.000000	79.4
ndhK-ndhC_p3	ndhK-ndhC	3	ACCATAGAGAAGCGGCCATA	CGAAAAGGGGCATTGGAATG	20	20	50.0	50.0	58.3	57.7	2.2	0.0	0.581379	51966	52025	203-209	1.000000	78.8
ndhK-ndhC_p4	ndhK-ndhC	4	ACCATAGAGAAGCGGCCATA	TGGGCAATGAGTTTCGATGT	20	20	50.0	45.0	58.3	57.8	2.2	0.0	0.481795	51966	52025	296-302	1.000000	78.6
ndhK-ndhC_p5	ndhK-ndhC	5	ACCATAGAGAAGCGGCCATA	AAAGGGGCATTGGAATGGTC	20	20	50.0	50.0	58.3	58.4	2.2	1.7	0.711014	51966	52025	200-206	1.000000	77.9
rbcL-accD_p1	rbcL-accD	1	GCACGACTACTTAACAGGGG	CTGAAAGCGAGACCACTACC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.008301	58637	60335	1855-2569	1.000000	91.9
rbcL-accD_p2	rbcL-accD	2	CGTAGCTAATCGAGTCGCTC	CTGAAAGCGAGACCACTACC	20	20	55.0	55.0	58.1	58.0	15.5	0.0	0.062362	58637	60335	1402-2116	1.000000	91.5
rbcL-accD_p3	rbcL-accD	3	GCTCTGACCGAGATCTTTGG	CTGAAAGCGAGACCACTACC	20	20	55.0	55.0	58.1	58.0	12.2	0.0	0.069084	58637	60335	1488-2202	1.000000	91.4
rbcL-accD_p4	rbcL-accD	4	AGATTGGGTCTCTCTACCGG	CTGAAAGCGAGACCACTACC	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.077059	58637	60335	1555-2269	1.000000	91.4
rbcL-accD_p5	rbcL-accD	5	GCACGACTACTTAACAGGGG	ACAAGAACAACACTGCCCAT	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.077748	58637	60335	1672-2386	0.500000	66.0
accD_p1	accD	1	GGTAGTGGTCTCGCTTTCAG	CCCATTGCTACGGGAATACC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.110121	60744	60863	923-2114	0.750000	81.1
accD_p2	accD	2	ACAGGTCTACAGGGGTACAC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.1	58.0	0.0	26.9	0.092594	60336	61979	2780-2780	0.250000	49.3
accD_p3	accD	3	GTTACAGGGGTACAGGGCTA	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.1	58.0	0.0	26.9	0.156939	60336	61979	2971-2971	0.250000	48.7
accD_p4	accD	4	TTACAGGGGTACAGGGCTAC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.1	58.0	0.0	26.9	0.156939	60336	61979	2970-2970	0.250000	48.7
accD_p5	accD	5	ACAGGTCTACAGGGGTACAC	CGGGAGGAGGAAATTCGTTT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.175015	60336	61979	2244-2244	0.250000	48.6
accD-psaI_p1	accD-psaI	1	GGTGACAGCCAGTTTTGGTA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	61980	62711	867-1056	1.000000	88.8
accD-psaI_p2	accD-psaI	2	ACATCTCCTACTACCGGTGG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	57.9	58.0	19.9	26.9	0.095838	61980	62711	887-1076	1.000000	88.4
accD-psaI_p3	accD-psaI	3	GGTGACAGCCAGTTTTGGTA	TTGTTTTTCGAGACCCCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.065591	61980	62711	1407-1596	1.000000	87.5
accD-psaI_p4	accD-psaI	4	GGTCCACGGTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.049947	61980	62711	657-845	0.750000	78.1
accD-psaI_p5	accD-psaI	5	GGTCCACGGTTTCTTTCCTT	TTGTTTTTCGAGACCCCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.064195	61980	62711	1197-1386	0.750000	76.6
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	63820	64772	626-1070	1.000000	84.5
ycf4-cemA_p2	ycf4-cemA	2	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	63820	64772	640-1084	1.000000	83.9
ycf4-cemA_p3	ycf4-cemA	3	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	63820	64772	638-1082	1.000000	83.5
ycf4-cemA_p4	ycf4-cemA	4	TTGGCCTATTTCTTGCGTGT	ATCCACCAGGGCAAAAAGAC	20	20	45.0	50.0	58.1	58.4	3.3	0.0	0.463125	63820	64772	621-1065	1.000000	82.2
ycf4-cemA_p5	ycf4-cemA	5	GGCCATTCCTTTGACTCGTA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.473234	63820	64772	693-1137	1.000000	82.0
trnP-GGG-psaJ_p1	trnP-GGG-psaJ	1	AAAACAAACGCGCTACCAAG	GGAGAGATATGTTTTTAGATCTCGC	20	25	45.0	40.0	57.9	57.4	5.7	0.0	5.689243	70446	70940	513-587	1.000000	52.0
trnP-GGG-psaJ_p2	trnP-GGG-psaJ	2	CAAAACAAACGCGCTACCAA	GGAGAGATATGTTTTTAGATCTCGC	20	25	45.0	40.0	58.2	57.4	5.7	0.0	5.746501	70446	70940	514-588	1.000000	52.0
trnP-GGG-psaJ_p3	trnP-GGG-psaJ	3	TTGTACCCAAAACAAACGCG	GGAGAGATATGTTTTTAGATCTCGC	20	25	45.0	40.0	57.8	57.4	3.5	0.0	5.765657	70446	70940	521-595	1.000000	52.0
trnP-GGG-psaJ_p4	trnP-GGG-psaJ	4	TACCCAAAACAAACGCGCTA	GGAGAGATATGTTTTTAGATCTCGC	20	25	45.0	40.0	58.4	57.4	5.7	0.0	5.984400	70446	70940	518-592	1.000000	52.0
trnP-GGG-psaJ_p5	trnP-GGG-psaJ	5	AAAACAAACGCGCTACCAAG	GAGAGATATGTTTTTAGATCTCGCA	20	25	45.0	36.0	57.9	56.8	5.7	0.0	6.283421	70446	70940	512-586	1.000000	52.0
infA-rps8_p1	infA-rps8	1	AATGAACGCGGAACATACCA	GCTGCAGTCCTCATTGATCA	20	20	45.0	50.0	57.9	58.0	12.5	16.6	0.136174	82952	83140	926-985	1.000000	85.8
infA-rps8_p2	infA-rps8	2	TGAACGCGGAACATACCATT	GCTGCAGTCCTCATTGATCA	20	20	45.0	50.0	57.9	58.0	12.5	16.6	0.136174	82952	83140	924-983	1.000000	85.8
infA-rps8_p3	infA-rps8	3	TGAACGCGGAACATACCATT	TGCTGCAGTCCTCATTGATC	20	20	45.0	50.0	57.9	58.0	12.5	16.6	0.136174	82952	83140	925-984	1.000000	85.8
infA-rps8_p4	infA-rps8	4	TTTCGAGTCCTTGTTGCGAA	GCTGCAGTCCTCATTGATCA	20	20	45.0	50.0	58.1	58.0	0.0	16.6	0.091427	82952	83140	1081-1140	0.500000	63.6
infA-rps8_p5	infA-rps8	5	TTTCGAGTCCTTGTTGCGAA	TGCTGCAGTCCTCATTGATC	20	20	45.0	50.0	58.1	58.0	0.0	16.6	0.091427	82952	83140	1082-1141	0.500000	63.6
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	TACCGCTAAGCTATTGGGGA	AACCAAAATCCCCAACACGA	20	20	50.0	45.0	57.9	57.9	18.7	0.0	0.165827	111777	112618	1427-1714	1.000000	87.5
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	CCATTCGACCATATCCGCAT	AACCAAAATCCCCAACACGA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.173870	111777	112618	1600-2217	1.000000	86.8
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CTCGACGCCAGGATGTTAAA	AAGCCCACATACGACGAAAA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.177434	111777	112618	1581-2198	1.000000	86.7
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	CTCGACGCCAGGATGTTAAA	AACCAAAATCCCCAACACGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.079311	111777	112618	2022-2639	1.000000	86.1
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CACATTCTGGTGTCCTAGGC	AACCAAAATCCCCAACACGA	20	20	55.0	45.0	58.0	57.9	3.0	0.0	0.104567	111777	112618	2131-2748	1.000000	85.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGTGCGACAAACAAATAAGTCA	AGCGTGTCTACCGATTTCAC	22	20	36.4	50.0	57.1	58.0	0.0	0.0	2.892289	115163	115966	829-889	1.000000	64.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGTGCGACAAACAAATAAGTCA	CAGCGTGTCTACCGATTTCA	22	20	36.4	50.0	57.1	58.0	0.0	0.0	2.893659	115163	115966	830-890	1.000000	64.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGTGCGACAAACAAATAAGTCA	TCCTAAGAGCAGCGTGTCTA	22	20	36.4	50.0	57.1	57.9	0.0	0.0	3.008735	115163	115966	839-899	1.000000	63.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGTGCGACAAACAAATAAGTCA	CCTAAGAGCAGCGTGTCTAC	22	20	36.4	55.0	57.1	57.8	0.0	0.0	3.080753	115163	115966	838-898	1.000000	62.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGTGCGACAAACAAATAAGTCA	CTAAGAGCAGCGTGTCTACC	22	20	36.4	55.0	57.1	57.8	0.0	0.0	3.080753	115163	115966	837-897	1.000000	62.5
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	ATGAACATCACGAGCTGTCC	CTCGACGCCAGGATGTTAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020318	130889	131960	1867-2470	1.000000	91.8
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	GGACCCTATGCAGAATGTGG	CTCGACGCCAGGATGTTAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.031710	130889	131960	1766-2369	1.000000	91.7
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	ATGAACATCACGAGCTGTCC	CACATTCTGGTGTCCTAGGC	20	20	50.0	55.0	58.0	58.0	0.0	3.0	0.045574	130889	131960	1976-2579	1.000000	91.6
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	TGTCATTATTCCCGCGGAAG	CTCGACGCCAGGATGTTAAA	20	20	50.0	50.0	58.1	58.0	30.7	0.0	0.056478	130889	131960	1974-2577	1.000000	91.5
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GGACCCTATGCAGAATGTGG	CACATTCTGGTGTCCTAGGC	20	20	55.0	55.0	58.0	58.0	0.0	3.0	0.056966	130889	131960	1875-2478	1.000000	91.5
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	ATACCCACACCAGCCTTTTG	CTCGACGCCAGGATGTTAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.081227	132033	132237	1004-1455	0.750000	72.3
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	ATGGCTATTGGTCGAACGAC	CTCGACGCCAGGATGTTAAA	20	20	50.0	50.0	58.1	58.0	17.3	0.0	0.068650	132033	132237	1233-1540	0.500000	62.5
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CTTCTTCCTCTTCAACGCGA	CTCGACGCCAGGATGTTAAA	20	20	50.0	50.0	57.9	58.0	15.9	0.0	0.070575	132033	132237	1136-1442	0.500000	61.9
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	TCTTCTTCCTCTTCAACGCG	CTCGACGCCAGGATGTTAAA	20	20	50.0	50.0	57.9	58.0	3.5	0.0	0.070575	132033	132237	1137-1443	0.500000	61.9
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	ATGGCTATTGGTCGAACGAC	CCTTGTTCCTACATGACCCG	20	20	50.0	55.0	58.1	58.0	17.3	0.0	0.088243	132033	132237	1565-1872	0.500000	61.8