Hotspot

rpl32-trnL-UAG

SSC · 104870-105389 · 520 bp

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Pi 0.0199
MarkerSeek score 66.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
520
variable_sites
44
pi
0.0199
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTAACGAGCATCCAAAACC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.1 57.9 592-604 bp 1.0 78.9
rpl32-trnL-UAG_p2 GGTAACGAGCATCCAAAACC AGCAGCGTGTCTACCAATTT 50.0 45.0 57.1 57.8 585-597 bp 1.0 78.5
rpl32-trnL-UAG_p3 GGTAACGAGCATCCAAAACC CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.1 57.8 591-603 bp 1.0 78.4
rpl32-trnL-UAG_p4 GGTAACGAGCATCCAAAACC CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.1 57.8 590-602 bp 1.0 78.4
rpl32-trnL-UAG_p5 GGTAACGAGCATCCAAAACC GCTTGAGCACTGCTTCCTAA 50.0 50.0 57.1 58.2 606-618 bp 1.0 78.3