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rbcL-psaI

LSC · 56478-57358 · 881 bp

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Pi 0.00772

MarkerSeek score 62.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 34
pi: 0.00772
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAGCCGGTAGATACCATCGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	974-1007 bp	1.0	82.8
rbcL-psaI_p2	ACATTTGACTTCAAGCCGGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	986-1019 bp	1.0	82.7
rbcL-psaI_p3	CAAGCCGGTAGATACCATCG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	975-1008 bp	1.0	82.4
rbcL-psaI_p4	AAGCCGGTAGATACCATCGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	980-1013 bp	1.0	82.2
rbcL-psaI_p5	ACATTTGACTTCAAGCCGGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	992-1025 bp	1.0	82.1