Hotspot

ndhC-trnV-UAC

LSC · 50351-51299 · 949 bp

Back to Triodia

Pi 0.00562

MarkerSeek score 68.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 28
pi: 0.00562
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	1049-1128 bp	1.0	80.6
ndhC-trnV-UAC_p2	TCCTTCACTAATCGGGGCTA	AGCCCAATGCAGAAAAATGC	50.0	45.0	57.5	57.9	1142-1221 bp	1.0	80.0
ndhC-trnV-UAC_p3	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.4	57.9	1053-1132 bp	1.0	79.5
ndhC-trnV-UAC_p4	CTGGTCCTTCACTAATCGGG	AGCCCAATGCAGAAAAATGC	55.0	45.0	57.4	57.9	1146-1225 bp	1.0	78.9
ndhC-trnV-UAC_p5	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.5	58.3	1050-1129 bp	1.0	78.8