Hotspot

psaA-ycf3

LSC · 42143-42770 · 628 bp

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Pi 0.00366

MarkerSeek score 61.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 628
variable_sites: 14
pi: 0.00366
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TATAGCGCTTACTCCGGGAA	55.0	50.0	58.0	58.0	751-829 bp	1.0	85.5
psaA-ycf3_p2	GGGGCCCTTAGCTATTGTTC	GCGCTTACTCCGGGAAATTA	55.0	50.0	58.0	58.1	747-825 bp	1.0	85.2
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	716-794 bp	1.0	84.9
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	712-790 bp	1.0	84.6
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	714-792 bp	1.0	83.8