Hotspot

psbM-petN

LSC · 17226-18029 · 804 bp

Back to Triodia

Pi 0.00892

MarkerSeek score 59.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 36
pi: 0.00892
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	AGTATGGGGGAGAAGTGGAC	40.0	55.0	56.7	58.1	849-889 bp	1.0	73.0
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.7	58.1	848-888 bp	1.0	73.0
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TATGGGGGAGAAGTGGACTC	40.0	55.0	56.7	57.8	847-887 bp	1.0	72.5
psbM-petN_p4	AAAACAGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.7	58.2	844-884 bp	1.0	72.2
psbM-petN_p5	AACAGCCAGCCAAAATGATT	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.5	58.1	846-886 bp	1.0	71.1