Hotspot

rps16-trnQ-UUG

LSC · 5612-6870 · 1259 bp

Back to Triodia

Pi 0.0089

MarkerSeek score 68.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1259
variable_sites: 62
pi: 0.0089
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1149-1338 bp	1.0	86.0
rps16-trnQ-UUG_p2	TCATCTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1160-1349 bp	1.0	84.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1153-1342 bp	1.0	81.3
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CGCATAAAACGAAATGGGGC	45.0	50.0	58.1	58.4	1211-1400 bp	0.933333	79.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GCATAAAACGAAATGGGGCG	45.0	50.0	58.1	58.4	1210-1399 bp	0.933333	79.9