Hotspot

psaC-ndhE

SSC · 106855-107350 · 496 bp

Back to Triniochloa

Pi 0.0202

MarkerSeek score 66.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 496
variable_sites: 10
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTTTACATCCATCCCAGGG	AGCCGCTATTGGACTATCCA	55.0	50.0	58.0	58.3	691-700 bp	1.0	78.8
psaC-ndhE_p2	GGCTTTACATCCATCCCAGG	AGCCGCTATTGGACTATCCA	55.0	50.0	58.0	58.3	692-701 bp	1.0	78.8
psaC-ndhE_p3	GTTGGACAAGCTCGTACACA	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	652-661 bp	1.0	77.8
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	653-662 bp	1.0	77.8
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	AGCCGCTATTGGACTATCCA	50.0	50.0	57.9	58.3	654-663 bp	1.0	77.8