Hotspot

ndhC-trnV

LSC · 49214-50096 · 883 bp

Back to Triniochloa

Pi 0.00827

MarkerSeek score 67.50

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 7
pi: 0.00827
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	972-1009 bp	1.0	82.7
ndhC-trnV_p2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	973-1010 bp	1.0	80.8
ndhC-trnV_p3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	971-1008 bp	1.0	80.2
ndhC-trnV_p4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	974-1011 bp	1.0	79.1
ndhC-trnV_p5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	972-1009 bp	1.0	78.4