Hotspot

atpI-atpH

LSC · 31408-32014 · 607 bp

Back to Triniochloa

Pi 0.0036

MarkerSeek score 64.70

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 607
variable_sites: 2
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	45.0	50.0	57.9	58.1	646-697 bp	1.0	83.0
atpI-atpH_p2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	45.0	50.0	57.9	58.1	649-700 bp	1.0	83.0
atpI-atpH_p3	TTTTGCAACATTAGCCGCAG	AGCCAATCCAGCAGCAATAA	45.0	45.0	57.9	57.9	659-710 bp	1.0	82.5
atpI-atpH_p4	TTTTGCAACATTAGCCGCAG	GCAGTACCTTGACCAACTCC	45.0	55.0	57.9	58.2	703-754 bp	1.0	82.2
atpI-atpH_p5	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	45.0	45.0	57.9	57.8	636-687 bp	1.0	82.1