Hotspot

trnG-trnT

LSC · 13728-15024 · 1297 bp

Back to Triniochloa

Pi 0.00697

MarkerSeek score 63.20

Primer available yes

Label trnG-trnT

Feature Metadata

label_name: trnG-trnT
feature_type: igs
region: LSC
length_bp: 1297
variable_sites: 9
pi: 0.00697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnT_p1	ACGAATCACACTTTTACCACT	ACCATGGCATTACTCTAGCAC	38.1	47.6	55.5	57.8	1431-1434 bp	1.0	43.0
trnG-trnT_p2	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCATGGCA	38.1	47.6	55.5	57.8	1443-1446 bp	1.0	42.6
trnG-trnT_p3	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCATGGC	38.1	47.6	55.5	57.8	1444-1447 bp	1.0	42.6
trnG-trnT_p4	AGAACGAATCACACTTTTACCAC	ACCATGGCATTACTCTAGCAC	39.1	47.6	57.3	57.8	1434-1437 bp	1.0	41.9
trnG-trnT_p5	GAACGAATCACACTTTTACCACT	ACCATGGCATTACTCTAGCAC	39.1	47.6	57.3	57.8	1433-1436 bp	1.0	41.9