Hotspot

ndhF

SSC · 107636-109864 · 2229 bp

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Pi 0.0211

MarkerSeek score 54.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 47
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	CATTCCATTACCTGACGCGA	50.0	50.0	58.0	58.1	2967 bp	0.5	59.1
ndhF_p2	AAGAGGGATCCACCGAAGAA	CATTCCATTACCTGACGCGA	50.0	50.0	58.0	58.1	2959 bp	0.5	59.1
ndhF_p3	ACAGGAACAAGAGGGATCCA	GACGCGAGGGACTTTTAGTT	50.0	50.0	58.0	57.9	2954 bp	0.5	59.0
ndhF_p4	AAGAGGGATCCACCGAAGAA	GACGCGAGGGACTTTTAGTT	50.0	50.0	58.0	57.9	2946 bp	0.5	59.0
ndhF_p5	ACAGGAACAAGAGGGATCCA	ATTCCATTACCTGACGCGAG	50.0	50.0	58.0	57.8	2966 bp	0.5	57.8