Hotspot

clpP1

LSC · 68710-70761 · 2052 bp

Back to Trimezia

Pi 0.0294

MarkerSeek score 61.60

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2052
variable_sites: 59
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TTGAAGAGCGGGAGATTTCG	CCACGCCTTCATAACTCCAA	50.0	50.0	58.0	57.9	2930-2970 bp	1.0	83.0
clpP1_p2	TTGAAGAGCGGGAGATTTCG	CTCCCGAAATACTCCAACCG	50.0	55.0	58.0	58.1	2928 bp	0.5	59.4
clpP1_p3	TTGAAGAGCGGGAGATTTCG	ACATTGGATCAAGAACCGGG	50.0	50.0	58.0	57.9	2847 bp	0.5	58.9
clpP1_p4	TTGAAGAGCGGGAGATTTCG	CATTGGATCAAGAACCGGGT	50.0	50.0	58.0	57.9	2846 bp	0.5	58.9
clpP1_p5	TTGAAGAGCGGGAGATTTCG	ATACCAAGGCAAACCCATGG	50.0	50.0	58.0	58.1	2699 bp	0.5	58.8