Hotspot

psaC-ndhE

SSC · 115111-115473 · 363 bp

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Pi 0.0435

MarkerSeek score 73.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 363
variable_sites: 14
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTCGTACGCATTGAGTACA	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.1	58.0	469-506 bp	1.0	85.4
psaC-ndhE_p2	AGCTCGTACGCATTGAGTAC	GCTATTGGGCTGGCTATTGT	50.0	50.0	57.8	58.0	470-507 bp	1.0	84.7
psaC-ndhE_p3	TCGTACGCATTGAGTACACC	GCTATTGGGCTGGCTATTGT	50.0	50.0	57.7	58.0	467-504 bp	1.0	84.1
psaC-ndhE_p4	GCTCGTACGCATTGAGTACA	AGCTATTGGGCTGGCTATTG	50.0	50.0	58.1	57.7	470-507 bp	1.0	83.3
psaC-ndhE_p5	ATCTGTGGGACAAGCTCGTA	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.4	58.0	482-519 bp	1.0	82.6