Hotspot

rpl32-trnL-UAG

SSC · 110907-111871 · 965 bp

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Pi 0.0431
MarkerSeek score 76.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
965
variable_sites
40
pi
0.0431
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTGTGCGACAAACAAGCAAG AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 994-1017 bp 1.0 85.3
rpl32-trnL-UAG_p2 TTGTGCGACAAACAAGCAAG CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 995-1018 bp 1.0 85.3
rpl32-trnL-UAG_p3 TTGTGCGACAAACAAGCAAG TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 1004-1027 bp 1.0 84.3
rpl32-trnL-UAG_p4 TTGTGCGACAAACAAGCAAG CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 1003-1026 bp 1.0 83.8
rpl32-trnL-UAG_p5 TTGTGCGACAAACAAGCAAG CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 1002-1025 bp 1.0 83.8