Hotspot

ndhF-rpl32

SSC · 109865-110744 · 880 bp

Back to Trimezia

Pi 0.062

MarkerSeek score 77.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 880
variable_sites: 50
pi: 0.062
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCCGAAAACACCATAGCTA	CTTGCTTGTTTGTCGCACAA	50.0	45.0	58.0	58.1	1189-1207 bp	1.0	86.7
ndhF-rpl32_p2	AGATTGGCCGAAAACACCAT	CTTGCTTGTTTGTCGCACAA	45.0	45.0	58.1	58.1	1194-1212 bp	1.0	86.3
ndhF-rpl32_p3	ACACATTCCCACAAGTTCCC	CTTGCTTGTTTGTCGCACAA	50.0	45.0	58.0	58.1	1492-1510 bp	1.0	86.1
ndhF-rpl32_p4	CACATTCCCACAAGTTCCCA	CTTGCTTGTTTGTCGCACAA	50.0	45.0	58.0	58.1	1491-1509 bp	1.0	86.1
ndhF-rpl32_p5	TCGCGTGAACCAAAAACCTA	CTTGCTTGTTTGTCGCACAA	45.0	45.0	58.0	58.1	1525-1543 bp	1.0	85.6