Hotspot

rpl16-rps3

LSC · 81293-81444 · 152 bp

Back to Trimezia

Pi 0.0567

MarkerSeek score 76.60

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 8
pi: 0.0567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AGAGCGGGGTTCATTCTTTT	CCTCTACAAACAATTCGCGC	45.0	50.0	57.4	57.8	327-334 bp	1.0	78.6
rpl16-rps3_p2	AACGAGTCACACACTAAGCA	CCTCTACAAACAATTCGCGC	45.0	50.0	57.1	57.8	269-278 bp	1.0	77.2
rpl16-rps3_p3	ACGAGTCACACACTAAGCAT	CCTCTACAAACAATTCGCGC	45.0	50.0	56.9	57.8	268-277 bp	1.0	75.4
rpl16-rps3_p4	AGAGCGGGGTTCATTCTTTTT	CCTCTACAAACAATTCGCGC	42.9	50.0	58.1	57.8	327-334 bp	1.0	74.3
rpl16-rps3_p5	AACGAGTCACACACTAAGCAT	CCTCTACAAACAATTCGCGC	42.9	50.0	57.6	57.8	269-278 bp	1.0	73.2