Hotspot

accD-psaI

LSC · 57816-58430 · 615 bp

Back to Trimezia

Pi 0.0422

MarkerSeek score 67.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 20
pi: 0.0422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	742-874 bp	1.0	86.1
accD-psaI_p2	ACGGCCAGTTTTGGTATGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	787-919 bp	1.0	86.0
accD-psaI_p3	AACGGCCAGTTTTGGTATGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	788-920 bp	1.0	85.9
accD-psaI_p4	CTACAACCGGTGGAGTAACG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	804-936 bp	1.0	85.9
accD-psaI_p5	TGGGATCTGTAGTAGGCGAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1008-1140 bp	1.0	85.5