Hotspot

trnT-GGU-psbD

LSC · 31158-32262 · 1105 bp

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Pi 0.0386

MarkerSeek score 76.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1105
variable_sites: 40
pi: 0.0386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1209-1269 bp	1.0	83.8
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	50.0	55.0	57.9	57.7	1193-1253 bp	1.0	83.7
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1208-1268 bp	1.0	82.6
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1172-1232 bp	1.0	82.4
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.3	57.7	1156-1216 bp	1.0	82.4